BECAS
CANIZO Brenda Vanina
congresos y reuniones científicas
Título:
DIFFERENTIAL BIOCONCENTRATION OF CADMIUM IN TISSUES AND ENDOSYMBIONTS OF THE SENTINEL ORGANISM Pomacea canaliculata
Autor/es:
CAMPOY DIAZ, DANIELA; CANIZO, BRENDA V.; WUILLOUD, RODOLFO G; VEGA, ISRAEL A.
Lugar:
Mendoza
Reunión:
Congreso; XXXIV REunión Científica Anual de la Sociedad de Biología de Cuyo; 2016
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Two morphs of an endosymbiont (identified as C and K corpuscles) are found within cells of the digestive gland in P. canaliculata. Since previous evidence suggested that cadmium produced a decrease in the frequency of copulation and the number of egg masses, and an increase of non-embryonated eggs in this snail, experiments were launched to measure cadmium concentrations in both morphs of the symbiont, as well as in samples of digestive gland, kidney, uterus and testis. Snails were cultured in reconstituted water (prepared with American Society for Testing and Materials type I water) and then exposed to different concentrations of cadmium in the aquarium water (50, 250, and 500 ug/L) for 28 days. Graphite furnace atomic absorption spectroscopy (instrumental detection limit = 0.03 ug/L) was used to determine cadmium. This element was detected in all tissues and endosymbionts of all exposed snails. High levels of cadmium were found in the digestive gland (50 µg/L) and kidney (250 and 500 µg/L), while they were lower in testis and uterus. The bioaccumulation in the digestive gland was at the expense of both morphs of the endosymbiont. These observations indicate that, after the ingress in the snail, cadmium is distributed preferentially in the digestive gland (which includes the endocytobiont) and kidney of P. canaliculata. Bioaccumulation of cadmium in testis and uterus (which was much lower) could explain the deleterious effects in reproductive functions. Together, these results suggest that this freshwater snail may be used as a bioindicator of cadmium pollution in limnic environments.