INVESTIGADORES
CAMPERI Silvia Andrea
congresos y reuniones científicas
Título:
New antimicrobial peptides as potential candidates in the growth control of Botrytis cinerea
Autor/es:
A. CARDILLO; S. M. ROMERO; S. L. GIUDICESSI; M. C. MARTÍNEZ-CERON; S.A. CAMPERI
Lugar:
Sitges, Barcelona
Reunión:
Simposio; 36th European and 12th International Peptide Symposium; 2022
Institución organizadora:
European peptide society
Resumen:
Antimicrobial peptides (AMPs) are small-polycationic molecules present in the immune response. There are currently more than 3000 experimentally reported AMPs. Particularly, temporins, a small AMP family present in frogs, have been discovered as potential candidates for antimicrobial activity. Until now, temporins have only been used in the control of bacteria and yeasts. Here we analyzed the application of temporins for the control of the phytopathogenic fungus Botrytis cinerea for a potential application in crops control of agronomic interest. Peptides were synthesized in solid phase using Fmoc chemistry and the purity of the synthesis was analyzed by HPLC and MALDI MS. The first screening was carried out testing different concentrations of the peptides in Petri dishes with Potato Dextrose Agar medium (PDA, Oxoid). PDA plates were inoculated with 1 µl of the spore suspension and incubated in the dark for 3 days. The growth diameter of the fungus on the plate was measured and the percentage of inhibition was calculated with respect to a control with pure DMSO. Three peptide concentrations were tested: 10, 50 and 100 µg/mL, and each test was performed in triplicate. In a first screening, best results were obtained with two peptides found in bibliography which had the Ser-Leu-Leu sequence and a high percentage of hydrophobic amino acids in their structure. From these results, a second screening was carried out using 10 different temporins with the Ser-Leu-Leu sequence. A fungus growth inhibition between 40% and 60% was observed at a concentration of 100 µg/mL. Of these, two peptides that showed 55% and 57% of inhibition were temporins. Both temporins have a high content of Leucine and Isoleucine in their structure and a net charge of 0, together with a high hydrophobicity. These results showed more efficient natural alternatives in the control of this pathogen of agroindustrial interest.