STRESS PARAMETRES ANALYSIS IN COTTON PLANTS BY CHEMOMETRIC

C. A. MOLDES; O. F. LIMA FILHO; J. M. CAMIÑA; S. G. KIRIACHEK; L. MOLAS; S. M. TSAI

San Luis

Congreso; XXX Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

Stress parameters (antioxidant enzymes activity and lipid peroxidation) in cotton plants were analyzed by chemometric. Plant were grown in hydroponic culture and added with increasing dose of silicon (Si). Enzymatic activity of catalase, guaiacol peroxidase, ascorbate peroxidase and the lipid peroxidation were determined in leaves and roots. Data were analyzed by chemometric methods -principal component analysis (PCA), cluster analysis (CA) and linear discriminant analysis (LDA)-. The results showed that silicon affects the activity of GPOX, APX and CAT in roots and leaves, but lipid peroxidation was not affected in comparison to a control (i.e. without Si). Furthermore, chemometric analysis showed that the enzymatic activities in leaves and roots depend to silicon concentration; however, application of Si upper to 100 mg L-1 in hydroponic solution produced a unique grouping in PCA, CA and LDA. As conclusion, these results could indicate that the metabolism it is stabilized beyond 100 mg L-1 of Si and upper concentrations of Si did not induce change in plants.