Changes in amino acid profile in roots of glyphosate resistant and susceptible soybean (Glycine max) induced by foliar glyphosate application

C.A. MOLDES; M.A. CANTARELLI; J.M. CAMIÑA; S.M. TSAI; R.A. ANTUNEZ DE ACEVEDO

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 2017 vol. 65 p. 8823 - 8828

0021-8561

Amino acid profiles are useful to analyze the responses to glyphosate in susceptible andresistant soybean lines. Comparisons of profiles for 10 amino acids (Asp, Asn,Glu, Gln, Ser, His, Gly, Thr, Tyr, Leu) by HPLC in soybean roots were performedin two near isogenic pairs (four varieties). Foliar application of glyphosatewas made to soybean plants after 5 weeks of seeding. Roots of four varietieswere collected at 0 and 72 h after glyphosate application (AGA) for amino acidanalysis by HPLC. Univariate analysis showed a significant increase of severalamino acids in susceptible as well as resistant soybean lines; however, aminoacids from the major pathways of carbon (C) and nitrogen (N) metabolism, suchas Asp, Asn, Glu and Gln, and Ser, increased significantly in susceptible varieties at 72 h AGA. Multivariateanalysis using principal component analysis (2D PCA and 3D PCA) alloweddifferent groups to be identified and discriminated based on the soybeangenetic origin, showing the amino acid responses on susceptible and resistantvarieties. Based on the results, it is possible to infer that the increase ofAsn, Asp, Glu, Gln, and Ser in susceptible varieties would be related to thederegulation of C and N metabolism, as well as changes in the growth mechanismsregulated by Ser.