Antifungal activity of b-carbolines on Penicillium digitatum and Botrytis cinerea

GABRIELA M. OLMEDO; LUCIANA CERIONI; MICAELA M. GONZALEZ; FRANCO M. CABRERIZO; VIVIANA A. RAPISARDA; SABRINA I. VOLENTINI

FOOD MICROBIOLOGY

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2017 vol. 62 p. 9 - 14

0740-0020

b-carbolines(bCs) are alkaloids widely distributed in nature and have demonstrated diverseantimicrobial properties. Here, we tested invitro six bCs against Penicilliumdigitatum and Botrytis cinerea, causalagents of postharvest diseases in vegetables and fruit. Full aromatic bCs(harmine, harmol, norharmane and harmane) exhibited a marked inhibitory effecton germination at concentrations between 0.5 and 1 mM, while dihydro-bCs(harmalina and harmalol) only caused germination delay. Harmol showed thehighest inhibitory effect on both fungal isolates. After 24 h of exposure to 1mM harmol, B. cinerea conidia revealeda severe cellular damage, exhibiting disorganized cytoplasm and thickened cellwall. Conidia membrane permeabilization was detected in treatments with harmolat subinhibitory and inhibitory concentrations. This alkaloid also inhibited mycelialgrowth, preventing sporulation at the highest concentration tested. Theseresults indicate that harmol might be an interesting candidate as a newantifungal molecule to control causal agents of postharvest diseases.