Naringenin interferes with the dispersal of Staphylococcus aureus biofilms.

LOMBARTE SERRAT, A; DOTTO, CM; SULIGOY CM; CERVINI BOHM, G; SAENZ, D; SORDELLI DO; GIACOMODONATO MN; BUZZOLA, FR

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Staphylococcus aureus biofilms are recalcitrant to antibiotic treatmentleading to chronic infections. The final benefit to the biofilmdevelopment is the potential for seeding dispersal or cellular detachment.The dispersal mechanism requires an active agr quorumsensing system and occurs in a protease-dependent manner. Naringenin(N) is a natural flavanone with antioxidant and antimicrobialproperties. The aim of this study was to investigate the effect of N onS. aureus biofilm. S. aureus AR411 (osteomyelitis isolate related toUSA300 clone) and SH1000 laboratory strain were used. Citotoxicityusing LM2 cell line was determined by MTT assay. Bacteria weretreated with N before biofilm formation takes place (prior-to-exposure)and 24 h after biofilms were formed (post-exposure). Biofilmswere spectrophotometrically quantified. Inhibition of agr system wasmonitored fluorometrically. Proteolytic activity was assessed usingmilk agar plates. N (50 μg/ml) did not affect the epithelial cells viability.The ability to form biofilm by AR411 strain (prior-to exposure)significantly diminished in presence of N (AR411: 3.27±0.11 vsAR411+N: 1.64±0.08, p