INVESTIGADORES
BUZZOLA Fernanda Roxana
artículos
Título:
Salicylic acid stabilizes Staphylococcus aureus biofilm by impairing the agr quorum-sensing system.
Autor/es:
DOTTO, CM; LOMBARTE SERRAT, A; LEDESMA, M; VAY, C; EHLING-SCHULZ, M; SORDELLI, DO; GRUNERT, T; BUZZOLA, FR
Revista:
Scientific Reports
Editorial:
Springer Nature
Referencias:
Año: 2021
Resumen:
Salicylic acid (SAL) has recently been shown to induce biofilm formation in Staphylococcus aureus and to affect the expression of virulence factors. This study was aimed to investigate the effect of SAL on the regulatory agr system and its impact on S. aureus biofilm formation. The agr quorum-sensing system, which is a central regulator in S. aureus pathogenicity, plays a pivotal role in the dispersal of S. aureus mature biofilms and contributes to the creation of new colonization sites. Here, we demonstrate that SAL impairs biofilm dispersal by interfering with agr expression. As revealed by our work, protease and surfactant molecule production is diminished, and bacterial cell autolysis is also negatively affected by SAL. Furthermore, as a consequence of SAL treatment, the S. aureus biofilm matrix revealed the lack of extracellular DNA. In silico docking and simulation of molecular dynamics provided evidence for a potential interaction of AgrA and SAL, resulting in reduced activity of the agr system. In conclusion, SAL stabilized the mature S. aureus biofilms, which may prevent bacterial cell dissemination. However, it may foster the establishment of infections locally and consequently increase bacterial persistence leading to therapeutic failure.