INVESTIGADORES
BUZZOLA Fernanda Roxana
artículos
Título:
Attenuated mutants of Staphylococus aureus with two temperature-sensitive lesions: isolation and characterization
Autor/es:
SORDELLI DO, CERQUETTI MC, BUZZOLA FR, GARCÍA VE, GOMEZ MI AND MORRIS-HOOKE A
Revista:
MICROBIOS
Editorial:
Cambridge, Eng. Faculty Press
Referencias:
Año: 1998 p. 95 - 102
ISSN:
0026-2633
Resumen:
The feasibility of constructing attenuated mutants of Staphylococcus aureus with two temperature-sensitive (ts) lesions for ultimate development of a live-attenuated strain was investigated. Temperature-sensitive S. aureus strain G/1/2, which grows well at 31 degrees C but does not replicate at 37 degrees C, was subjected to chemical mutagenesis. After two enrichment cycles, fifteen mutants able to grow at 25 degrees C but unable to grow at 31 degrees C, were identified. Growth curves with temperature shifts from 25 to 31 degrees C, and from 31 to 37 degrees C confirmed that these were mutants with two lesions (dts), each with a different cut-off temperature. The reversion frequency of mutant G/1/2 at 37 degrees C was 2 x 10(-6) whereas those of several dts mutants were much lower (dts7: 7 x 10(-9) and dts12: 1 x 10(-9)). There was no increase in ts mutation reversion rate in response to prolonged incubation at 37 degrees C. The data support the further development of these mutants for use as a stable attenuated vaccine