INVESTIGADORES
BOUZAT Cecilia Beatriz
congresos y reuniones científicas
Título:
Flavonoids as allosteric Modulators of alpha7 nicotinic receptor
Autor/es:
NIELSEN, B.E.; BOUZAT, C.
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
Ubiquitously present in plants, flavonoids are a class of polyphenolic compounds comprising a benzopyrone moiety. These compounds have been reported to decline incidence rate and development of several neurodegenerative disorders, such as Alzheimer?s and Parkinson?s diseases. Two flavonoids, genistein and quercetin, have been also described as type I positive allosteric modulators (PAMs) of α7 nicotinic acetylcholine receptor, which increase the amplitude of macroscopic responses evoked by ACh without changing the desensitization rate. The molecular mechanism underlying these macroscopic effects remains unknown. Our main objective is to unravel the molecular basis of flavonoid allosteric potentiation of α7 at the single-channel level. Receptors were expressed in mammalian cells and receptor function was evaluated by patch-clamp recordings. We analyzed the concentration-dependent effects of a prototype flavonoid for each class: quercetin as flavones, genistein as isoflavones and 5,7-dihydroxy-4-phenylcoumarin as neoflavonoids. We here reported for the first time that neoflavonoids act as PAMs of α7. All flavonoids increased the mean open channel lifetime and the mean burst duration of α7, although the magnitude of the change differed among the different compounds. In order to define the structural determinants of potentiation, we evaluated flavonoid potentiation in an α7 receptor carrying a quintuple mutation at the transmembrane region that makes it insensitive to potentiation by type II PAMs and in a chimeric α7-5HT3A receptor with the extracellular domain of α7 and the transmembrane region of 5-HT3A. We conclude that, in addition to the well-known effects as antioxidants, the unique properties of flavonoids as natural α7 PAMs make them candidate drugs for the treatment of different neurodegenerative disorders. Keywords: flavonoids, alpha 7 nicotinic receptor, positive allosteric modulators, patch-clamp.