INVESTIGADORES
BIANCO Maria Isabel
congresos y reuniones científicas
Título:
Clostridium botulinum spores load in soil of Chubut and Río Negro, Argetnina
Autor/es:
DE JONG LIT, CICCARELLI AS, BIANCO MI, DEGARBO SM, CABALLERO PA, CICCARELLI MA, FERNÁNDEZ RA.
Lugar:
San Luis, Argentina
Reunión:
Otro; XXII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2004
Resumen:
Clostridium botulinum (Cb) spores are widely distributed in nature. They occur in both cultivated and forest soils, and can often be found on the environmental dust, many agricultural products, honey, and medicinal plants. Frequency of positive soil samples for Cb and the spore load can be considered two important parameters for botulism transmission in its forms: foodborne botulism, infant botulism, and wound botulism. Forty nine soil samples from Chubut (25) and Río Negro (24) were proceeded for detection of Cb viable spores concentration (VSC) with the most probable number (MPN) method. Chubut: Positive results were obtained in 4/25 (15.66%) samples. VSC per g of soil (MPN/g): 84% (21/25) with less than 0.11 (0.02-0.21)* MPN/g; 4% (1/25) with 0.12 (0.04-0.41)* MPN/g; 8% (8/25) with 0.33 (0.10-1.09)* and 4% (1/25) with 1.83% (0.55-6.05)* MPN/g. BoNT types: A 50% (2/4), B 25% (1/4), and E 25% (1/4). Río Negro: Positive results were obtained in 4/24 (16.66%) samples. VSC: 83.33% (21/24) with less than 0.11 (0.02-0.21)* MPN/g; and the other with 0.12 (0.04-0.41)*; 0.26 (0.08-0.86)*; 0.73 (0.22-2.41)*, and 1.83% (0.55-6.05)* MPN/g. BoNT types: A 50% (2/4), B 50% (2/4) [*95% confidence limits]. Results are similar in both province but they are lower than those obtained in other regions of Argentina, with high incidence of human botulism