INVESTIGADORES
BELTRAMINO Ariel Anibal
congresos y reuniones científicas
Título:
Secondary structure of the partial mitochondrial large ribosomal subunit of Omalonyx unguis (d´Orbigny, 1837) (Gastropoda, Succineidae)
Autor/es:
GUZMÁN, L.B.; SERNIOTTI, E.N.; VOGLER, R.E.; BELTRAMINO, A.A.; RUMI, A.; PESO, J.G.
Lugar:
Foz do Iguaçu
Reunión:
Congreso; XXII International Congress of Genetics; 2018
Institución organizadora:
Sociedade Brasileira de Genética
Resumen:
Molecular markers as tools for identification and description of genetic variability are widely disseminated in gastropod research, due to its relatively simplicity, accuracy, informativeness and reproducibility. Among the mitochondrial markers commonly used for such purposes, ribosomal RNA genes have received considerable attention. These gene sequences are subject to frequent insertion-deletion events and are folded into complex secondary structures. Although ribosomal regions usually show high rates of variation, multiple alignment and comparison of sequences can be overcome through structural analyses. This allows assessing the structural and functional restrictions of genetic variation, where the loops of the molecules usually accumulate the greatest variation, while the stems are particularly well conserved. In this work, we present a structural model involving domains IV and V of the large ribosomal subunit gene (16S-rRNA) of Omalonyx unguis (d´Orbigny, 1837), a semi-slug species inhabiting the Paraná River basin. This species belongs to Succineidae, a family comprising a few South American representatives whose species-specific identification by anatomical features is difficult. DNA extraction was performed from the muscle foot from an individual from the Garupá Stream, Misiones Province, Argentina by using a classic CTAB protocol. PCR amplification was performed by means of universal primers. After purification, both DNA strands were sequenced and subsequently edited, resulting in 258 bp fragment. A secondary structure model was manually derived by comparison with structural reference models for other molluscs. Such structural model is the first available for the Succineidae and is expected to allow further comparative analyses of structural changes within the family.