INVESTIGADORES
BELFORTE Juan Emilio
artículos
Título:
Modulation of Tau Isoforms Imbalance Precludes Tau Pathology and Cognitive Decline in a Mouse Model of Tauopathy
Autor/es:
ESPÍNDOLA, SONIA LORENA; DAMIANICH, ANA; ALVAREZ, RODRIGO JAVIER; SARTOR, MANUELA; BELFORTE, JUAN EMILIO; FERRARIO, JUAN ESTEBAN; GALLO, JEAN-MARC; AVALE, MARÍA ELENA
Revista:
Cell Reports
Editorial:
Elsevier Inc.
Referencias:
Año: 2018 vol. 23 p. 709 - 715
ISSN:
2211-1247
Resumen:
The microtubule-associated protein tau regulates myriad neuronal functions, such as microtubule dynamics, axonal transport and neurite outgrowth. Tauopathies are neurodegenerative disorders characterized by the abnormal metabolism of tau, which accumulates as insoluble neuronal deposits. The adult human brain contains equal amounts of tau isoforms with three (3R) or four (4R) repeats of microtubule-binding domains, derived from the alternative splicing of exon 10 (E10) in the tau transcript. Several tauopathies are associated with imbalances of tau isoforms, due to splicing deficits. Here, we used a trans-splicing strategy to shift the inclusion of E10 in a mouse model of tauopathy that produces abnormal excess of 3R tau. Modulating the 3R/4R ratio in the prefrontal cortex led to a significant reduction of pathological tau accumulation concomitant with improvement of neuronal firing and reduction of cognitive impairments. Our results suggest promising potential for the use of RNA reprogramming in human neurodegenerative diseases