INVESTIGADORES
BELAUNZARAN maria Laura
congresos y reuniones científicas
Título:
LIPIDS FROM LEISHMANIA AMAZONENSIS AND LEISHMANIA BRAZILIENSIS PROMASTIGOTES PROMOTE MACROPHAGE POLARIZATION
Autor/es:
LOPEZ, SEBASTIAN ANDRES; PENAS, FEDERICO N; LAUTHIER, JUAN JOSE; CADENA, MARIA F; GOREN, NORA B; RUYBAL, PAULA; GIMÉNEZ, GUADALUPE; BELAUNZARÁN, MARIA LAURA
Reunión:
Simposio; Host-Pathogen Interaction Meeting 2022 - Molecular and cellular mechanisms of Host Pathogen Interaction; 2022
Institución organizadora:
Instituto Butantan
Resumen:
INTRODUCTION:Macrophages (Mo) can be activated via classical (M1) andalternative (M2) activation. Control of Leishmania spp. infection requires a Th1response & M1Mo activation. However, Th2 response and M2Mo activation canlead to parasite establishment in the host cell.OBJECTIVES:This work analyzesthe biological effects of L.amazonensis (Lama) and L.braziliensis (Lbra) lipidsfrom lysed promastigotes in murine peritoneal Moactivation.METHODS:L.braziliensis M2904 & L.amazonensis PH8promastigotes strains were axenically cultured in supplemented RPMI mediumat 24oC. Peritoneal Mo were obtained from BALB/c mice. Briefly, parasites weredisrupted, total lipids were extracted according to Bligh & Dyer and suspendedin ethanol for Mo stimulation. Lipid bodies (LB), Nitric Oxide (NO), Arginaseactivity (Aa) & M1/M2 markers mRNA were determined as described (Bott et al.,2018; Penas et al., 2017).RESULTS:Lama & Lbra induced a significant numberof LB, with respect to control (~10.7 & 10.3 vs 4.5 LB/Mo). In relation toinducible nitric oxide synthase (iNOS) activity, associated with M1Mo activation,Lama induced significant NO release (32.3 μM), as determined by the Griessreaction. However, Lbra (14μM) showed no significant differences in NOrelease when compared to control (10.4 μM). Moreover, Lama & Lbra inducedsignificant Aa, M2Mo marker, with respect to control. Lbra induced the highestvalues of Aa followed by Lama (~14 & 7.5 vs 1.7 μg urea/μg protein/h). Finally,we determined by RT–qPCR that Lama induced higher levels of TNFα mRNAexpression (M1Mo marker) than Lbra. In contrast, Lbra induced a significantlevel of IL-10 mRNA expression, but Lama showed no significant differenceswith respect to control. As regards other M2Mo markers, we observed that Lbra& Lama induced significant levels of FIZZ1 mRNA expression (~20.3 & 24 timesvs control) and in the case of YM1 mRNA expression both Lbra & Lamashowed significant levels (~4.8 & 7.9 times vs. control).CONCLUSION:L.amazonensis and L.braziliensis promastigotes possess bioactive lipids whichcan modulate macrophages responses and might contribute for parasite control/survival. Granted by UBA/CONICET/FONCyT