INVESTIGADORES
BELAUNZARAN maria Laura
artículos
Título:
Babesia bovis: lipids from virulent S2P and attenuated R1A strains trigger differential signalling and inflammatory responses in bovine macrophages
Autor/es:
GIMÉNEZ, GUADALUPE; BELAUNZARÁN, MARIA LAURA; PONCINI, CAROLINA V; BLANCO, F C; ECHAIDE, IGNACIO; ZAMORANO , P; LAMMEL, ESTELA MARÍA; GONZÁLEZ CAPPA, STELLA MARIS; DURANTE DE ISOLA, ELVIRA LUISA
Revista:
PARASITOLOGY
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Lugar: Cambridge; Año: 2013 vol. 140 p. 530 - 540
ISSN:
0031-1820
Resumen:
The intra-erythrocytic protozoan Babesia bovis is an economically important pathogen that causes an acute and often fatal infection in adult cattle. Babesiosis limitation depends on the early activation of macrophages, essential cells of the host innate immunity, which can generate an inflammatory response mediated by cytokines and nitric oxide (NO). Herein, we demonstrate in bovine macrophages that lipids from B. bovis attenuated R1A strain (LA) produced a strongerNOrelease, an early TNFαmRNAinduction and 2-fold higher IL-12p35mRNAlevels compared to the lipids of virulent S2P strain (LV). Neither LA nor LV induced anti-inflammatory IL-10. Regarding signalling pathways, we here report that LA induced a significant phosphorylation of p38 and extracellular signal-regulated kinases 1 and 2 (ERK1/2) whereas LV only induced a reduced activation of ERK1/2. Besides, NF-κB was activated by LA and LV, but LA produced an early degradation of the inhibitor IκB. Interestingly, LV and the majority of its lipid fractions, exerted a significant inhibition of concanavalin A-induced peripheral blood mononuclear cell proliferation with respect to LA and its corresponding lipid fractions. In addition, we determined that animals infected with R1A developed a higher increase in IgM anti-phosphatidylcholine than those inoculated with S2P. Collectively, S2P lipids generated a decreased inflammatory response contributing to the evasion of innate immunity. Moreover, since R1A lipids induced a pro-inflammatory profile, we propose these molecules as good candidates for immunoprophylactic strategies against babesiosis.