Screening of xylanolytic bacteria using a colour plate method. Journal Applied Bacteriology. .

BRECCIA JD,; CASTRO GR,; BAIGORI MD,; SINERIZ F,

JOURNAL OF APPLIED BACTERIOLOGY

Blackwell-Wiley

Año: 1995 vol. 78 p. 469 - 472

0021-8847

A simple procedure is described for the screening and isolation of xylanolytic bacteria, able to degrade hemicellulose. Bagasse hemicellulose was purified, cross-linked with green dye for use as indicator, and used in a Petri dish assay for the determination of xylanolytic activity. Hydrolysis of the substrate causes the formation of transparent halos around bacterial colonies. The screening procedure was used to select thermoresistant bacteria. The four best isolates were characterized and classified as Bacillus species.