INVESTIGADORES
BRECCIA Javier Dario
artículos
Título:
Polyethyleneimine coating of magnetic particles increased the stability of an immobilized diglycosidase
Autor/es:
MINIG M; MAZZAFERRO LS; CAPECCE A.; BRECCIA JD
Revista:
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Editorial:
John Wiley and Sons - IUBMB
Referencias:
Lugar: Malden MA 02148, USA; Año: 2015 vol. 62 p. 94 - 100
ISSN:
0885-4513
Resumen:
The diglycosidase, α-rhamnosyl-β-glucosidase, from Acremonium sp. DSM24697 was immobilized by adsorption and cross-linking onto aniline-iron particles (PI). The immobilization yield was 31.2% with 8.9% immobilization efficiency, but the preparation loss stability in comparison with the soluble form of the enzyme in operational conditions at 60 °C. One parameter involved in the reduced stability of the heterogeneous preparation was the protein metal catalyzed oxidation achieved by iron traces supplied from the support. To overcome the harmful effect, iron particles were coated with polyethyleneimine (0.84 mg/g) previous to the immobilization of the catalyst. The increased stability of the catalyst was correlated with the amount of iron released from the support. In operational conditions, the uncoated particles lost between 76─52% activity after 2 cycles of reuse, while PEI-coated preparation reduced 45─28% activity after 5 cycles of reuses in the range of pH 5.0 to 10 respectively. Hence, polymer coating of magnetic materials used as enzymes supports might be an interesting approach to improve the performance of biotransformation processes.