GENETIC DIVERSITY OF SUGARCANE MOSAIC VIRUS COMPLEX IN TUCUMÁN, ARGENTINA

M.F. PERERA, M.P. FILIPPONE, J. RAMALLO, M.I. CUENYA, M.L. GARCÍA AND A.P. CASTAGNARO

Durban, SudÁfrica

Congreso; ISSCT 2007; 2007

ISSCT

SUGARCANE leaves with mosaic symptoms were collected in 2006 from six locations throughout Tucumán, Argentina, and analysed by the RT-PCR-RFLPs (Yang and Mirkov, 1997) and the nucleotide sequences of the coat protein (CP) genes amplified. The presence of the flexuous virions typical of the potyviruses was confirmed by the transmission electron microscopy in all samples. Using the primers SCMV R3/F4, a 900-bp fragment was amplified from 93% of samples and 33% of them had the E strain RFLP profile, while the rest of them produced nine different profiles that did not match with those of any known strains. Sequence analyses of the CP genes amplified from these samples with unknown RFLP profiles show that 20% of the samples with the unknown profiles was a strain close to the SCMV D strain, while the rest showed a great genetic diversity. The presence of SrMV was detected in 90% of the total samples, and most of these samples were also infected by SCMV, indicating that the co-existence of SCMV and SrMV is common in the region. The RFLP analysis determined the presence of SrMV strains SCM and SCI in 68% and 14% of the samples, respectively while, in approximately 18% of the cases, both the strains SCM and SCH were present. No RT-PCR product was produced by either SCMV or SrMV primer pair in one symptomatic sample, suggesting the presence of another pathogen that produces similar symptomatology. throughout Tucumán, Argentina, and analysed by the RT-PCR-RFLPs (Yang and Mirkov, 1997) and the nucleotide sequences of the coat protein (CP) genes amplified. The presence of the flexuous virions typical of the potyviruses was confirmed by the transmission electron microscopy in all samples. Using the primers SCMV R3/F4, a 900-bp fragment was amplified from 93% of samples and 33% of them had the E strain RFLP profile, while the rest of them produced nine different profiles that did not match with those of any known strains. Sequence analyses of the CP genes amplified from these samples with unknown RFLP profiles show that 20% of the samples with the unknown profiles was a strain close to the SCMV D strain, while the rest showed a great genetic diversity. The presence of SrMV was detected in 90% of the total samples, and most of these samples were also infected by SCMV, indicating that the co-existence of SCMV and SrMV is common in the region. The RFLP analysis determined the presence of SrMV strains SCM and SCI in 68% and 14% of the samples, respectively while, in approximately 18% of the cases, both the strains SCM and SCH were present. No RT-PCR product was produced by either SCMV or SrMV primer pair in one symptomatic sample, suggesting the presence of another pathogen that produces similar symptomatology. throughout Tucumán, Argentina, and analysed by the RT-PCR-RFLPs (Yang and Mirkov, 1997) and the nucleotide sequences of the coat protein (CP) genes amplified. The presence of the flexuous virions typical of the potyviruses was confirmed by the transmission electron microscopy in all samples. Using the primers SCMV R3/F4, a 900-bp fragment was amplified from 93% of samples and 33% of them had the E strain RFLP profile, while the rest of them produced nine different profiles that did not match with those of any known strains. Sequence analyses of the CP genes amplified from these samples with unknown RFLP profiles show that 20% of the samples with the unknown profiles was a strain close to the SCMV D strain, while the rest showed a great genetic diversity. The presence of SrMV was detected in 90% of the total samples, and most of these samples were also infected by SCMV, indicating that the co-existence of SCMV and SrMV is common in the region. The RFLP analysis determined the presence of SrMV strains SCM and SCI in 68% and 14% of the samples, respectively while, in approximately 18% of the cases, both the strains SCM and SCH were present. No RT-PCR product was produced by either SCMV or SrMV primer pair in one symptomatic sample, suggesting the presence of another pathogen that produces similar symptomatology. throughout Tucumán, Argentina, and analysed by the RT-PCR-RFLPs (Yang and Mirkov, 1997) and the nucleotide sequences of the coat protein (CP) genes amplified. The presence of the flexuous virions typical of the potyviruses was confirmed by the transmission electron microscopy in all samples. Using the primers SCMV R3/F4, a 900-bp fragment was amplified from 93% of samples and 33% of them had the E strain RFLP profile, while the rest of them produced nine different profiles that did not match with those of any known strains. Sequence analyses of the CP genes amplified from these samples with unknown RFLP profiles show that 20% of the samples with the unknown profiles was a strain close to the SCMV D strain, while the rest showed a great genetic diversity. The presence of SrMV was detected in 90% of the total samples, and most of these samples were also infected by SCMV, indicating that the co-existence of SCMV and SrMV is common in the region. The RFLP analysis determined the presence of SrMV strains SCM and SCI in 68% and 14% of the samples, respectively while, in approximately 18% of the cases, both the strains SCM and SCH were present. No RT-PCR product was produced by either SCMV or SrMV primer pair in one symptomatic sample, suggesting the presence of another pathogen that produces similar symptomatology. UGARCANE leaves with mosaic symptoms were collected in 2006 from six locations throughout Tucumán, Argentina, and analysed by the RT-PCR-RFLPs (Yang and Mirkov, 1997) and the nucleotide sequences of the coat protein (CP) genes amplified. The presence of the flexuous virions typical of the potyviruses was confirmed by the transmission electron microscopy in all samples. Using the primers SCMV R3/F4, a 900-bp fragment was amplified from 93% of samples and 33% of them had the E strain RFLP profile, while the rest of them produced nine different profiles that did not match with those of any known strains. Sequence analyses of the CP genes amplified from these samples with unknown RFLP profiles show that 20% of the samples with the unknown profiles was a strain close to the SCMV D strain, while the rest showed a great genetic diversity. The presence of SrMV was detected in 90% of the total samples, and most of these samples were also infected by SCMV, indicating that the co-existence of SCMV and SrMV is common in the region. The RFLP analysis determined the presence of SrMV strains SCM and SCI in 68% and 14% of the samples, respectively while, in approximately 18% of the cases, both the strains SCM and SCH were present. No RT-PCR product was produced by either SCMV or SrMV primer pair in one symptomatic sample, suggesting the presence of another pathogen that produces similar symptomatology.