INVESTIGADORES
CASTAGNARO atilio pedro
artículos
Título:
Production of healthy seed cane in Tucumán, Argentina.
Autor/es:
NOGUERA A.S.; PAZ N. DEL V.; DÍAZ M.E.; PERERA M.F.; DÍAZ ROMERO C.; GARCÍA M.B.; FILIPPONE, M.P.; WELIN B.; CUENYA M.I.; DIGONZELLI, P; CASTAGNARO A.P
Revista:
INTERNATIONAL SUGAR JOURNAL
Editorial:
INT SUGAR JOURNAL LTD
Referencias:
Año: 2015 p. 112 - 116
ISSN:
0020-8841
Resumen:
Since 2001, the ?Estación Experimental Agroindustrial Obispo Colombres? (EEAOC) has been working on the?Vitroplantas Project?. On average 70 000 sugarcane plantlets of commercial varieties are produced through invitro meristem culture in order to eliminate systemic diseases caused by bacteria and viruses. The sanitation ofplant material is achieved through in vitro culture of apical meristems from donor plants previously hot-watertreatedand grown for 3 years under greenhouse conditions with anti-aphid screens. Systemic diseases aredetected in both meristem donor plants and micropropagated plantlets using different molecular diagnostictechniques. It is well known that in vitro plant tissue culture can produce somaclonal variations, which consistof genetic changes in cultured cells and tissues. In order to guarantee that seedlings propagated in vitro areidentical to parental varieties, molecular markers to quantify and detect somaclonal variation are routinelyapplied. Thus, the aim of this project is to guarantee healthy and genetically pure plantlets before transfer tothe field for seed cane propagation. After propagation and testing, in vitro plantlets undergo an acclimatisationprocess in a specially adapted greenhouse at the EEAOC. In order to avoid dehydration of plantlets, theprocess takes place in a greenhouse with very high relative humidity (RH=80?100%) and low light intensity.After acclimatisation, two more stages of conventional field propagation (Basic and Registered), are carriedout before the seed cane is finally distributed among sugarcane growers. The implementation of the project?Vitroplantas? has greatly improved the health and biomass yields of sugarcane production in Tucuman,Argentina.