INVESTIGADORES
CASTAGNARO atilio pedro
artículos
Título:
Closely-related Xanthomonas citri subsp. citri isolates trigger distinct histological and transcriptional responses in Citrus limón
Autor/es:
ORCE, IG1; ; DEBES, M; SENDÍN, L; LUQUE, A; ARIAS, M; VOJNOV, A; MARANO, MR; CASTAGNARO, AP; FILIPPONE, MP
Revista:
SCIENTIA AGRICOLA
Editorial:
UNIV SAO PAOLO
Referencias:
Lugar: Piracicaba; Año: 2016
ISSN:
0103-9016
Resumen:
Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), has an important economic impact on citrus industry. Extensive information is available about disease, nevertheless the study of plant-pathogen interactions could provide new information in the understanding of citrus canker disease., but genetic bases promoting the disease are still unclear. A newisolate has been identified, Xcc AT33 , which has a high genetic similarity (>90%) to virulent34 Xcc T reference strain based on genetic clustering analyses of the rep-PCR fingerprinting35 patterns, but it does not produce cankerous lesions in a host-specific interaction with Citruslimon. In this study, we compared C. limon responses to Xcc AT 36 and to the virulent Xcc37 reference T strain at both histological and transcriptional levels. Histologically, leavesinoculated with Xcc AT 38 did not exhibited neither a typical disordering of the spongy39 mesophyll, nor a swelling of epidermisal. A particular content (undetermined) was also40 found in mesophyll cells near stomata, together with an increased starch accumulation. The41 transcriptomic profiles were compared by using cDNA-AFLP technique. A total of 12142 fragments derived from transcript (TDF) were either specifically induced or repressed by43 the isolates, and 62 were sequenced. Analysis of global expression identified different44 classes of genes known to be involved in plant?pathogen interactions. This work constitutesthe first approach in the study of the specific interaction between the avirulent Xcc AT 4546 isolate and C. limon.