INVESTIGADORES
CASTAGNARO atilio pedro
artículos
Título:
Enhanced resistance to Botrytis cinerea mediated by the transgenic expression
Autor/es:
GABRIEL R. VELLICCE, JUAN C. DIAZ RICCI, LAZARO HERNANDEZ & ATILIO P. CASTAGNARO
Revista:
TRANSGENIC RESEARCH
Editorial:
Springer
Referencias:
Año: 2006 p. 57 - 68
ISSN:
0962-8819
Resumen:
Plants of strawberry (cultivar Pa´ jaro) were transformed with three defense related genes: ch5B, gln2 andch5B, gln2 and ap24 using Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, whileusing Agrobacterium tumefaciens. The ch5B gene encodes for a chitinase from Phaseolus vulgaris, while gln2 and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward and ap24 encode for a glucanase and a thaumatin-like protein, respectively, both from Nicotiana tabacum. Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward . Sixteen transgenic lines expressing one or a combination of two defense genes were obtained. Phytopathological tests showed that two transgenic lines expressing only the ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward ch5B gene displayed high levels of resistance to gray mold disease (Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Botrytis cinerea). The resistance was correlated with the presence of the foreign protein CH5B and the increase of chitinolytic activity in leaves. However, resistance toward Colletotrichum acutatum, the etiological agent of the anthracnose disease, was not enhanced in the transgenic plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop. to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop. plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop. to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop. , the etiological agent of the anthracnose disease, was not enhanced in the transgenic plants. These results suggest that the ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop. to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop. ch5B gene can be used to introduce transgene-mediated resistance to gray mold in strawberry, due to the lack of natural resistance to this disease in the crop.