ARAMENDIA Pedro Francisco
congresos y reuniones científicas
Quantitative imaging in single molecule fluorescence localization
Congreso; LIV Reunión Anual de la Sociedad de Argentina de Investigaciones en Bioquímica y Biologia Molecular; 2018
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica
Fluorescence microscopy is a widely used technique to study cellularstructure and dynamics. The introduction of methods breaking thediffraction limit of optical resolution, named super resolutionmicroscopy or optical nanoscopy, allowed tens of nanomenter lateralresolution to provide structural details unattained before.These achievements posed new challenges for the design andphotochemical studies of fluorescent probes with specificity for acertain substrate, fluorophores whose fluorescence could be turned onand off, photochemical studies under high irradiation conditions.Soon it was also evident the need to introduce methods to analyzeimages to precisely locate single molecules and to evaluate molecularassociation.In this talk I will deal with two problems we are working at, usingoptical nanoscopy, in CIBION in collaboration with IBioBA (Institutode Biomedicina de Buenos Aires-MPSP).On one side we studied the GPCR membrane receptor corticotropinrelease hormone receptor 1 (CRHR1). For that, we designed andsynthesized a fluorescent probe that works as an antagonist anddeveloped a method to evaluate the molecular binding constant in thecellular environment.On the other side, we undertook the study of the interaction betweenHIF (Hypoxia inducible factor), VHL (von Hippel-Lindau complex),RSUME (RWD-domain-containing sumoylation enhancer) in the hypoxiaadaptation syndrome of cancer cells. In the beginning of this projectwe characterize the distribution of the proteins in the cell.p { margin-bottom: 0.25cm; direction: ltr; color: rgb(0, 0, 0); line-height: 120%; }p.western { font-family: "Liberation Serif", "Times New Roman", serif; font-size: 12pt; }p.cjk { font-family: "Droid Sans Fallback"; font-size: 12pt; }p.ctl { font-family: "FreeSans"; font-size: 12pt; }