INVESTIGADORES
ALARCON laureano Martin
artículos
Título:
Effect of cholesterol on the hydration properties of ester and ether lipid membrane interphases
Autor/es:
PÉREZ, H.A.; ALARCÓN, L.M.; VERDE, A.R.; APPIGNANESI, G.A.; GIMÉNEZ, R.E.; DISALVO, E.A.; FRÍAS, M.A.
Revista:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2021 vol. 1863
ISSN:
0005-2736
Resumen:
Fluorescence spectroscopy and Molecular Dynamics results show that cholesterol reduces water along the chains in ether lipids by changing the water distribution pattern between tightly and loosely bound water molecules. Water distribution was followed by emission spectra and generalized polarization of 6-dodecanoyl-2-dimethyl aminonaphthalene (Laurdan) inserted in 1,2-dimiristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-di-O-tetradecyl-sn-glycero-3-phosphocholine (14: 0 Diether PC) membranes. Molecular Dynamics simulations indicate that the action of cholesterol could be different in ether PC in comparison to ester PC. In addition, Cholesterol seems to act “per se” as an additional hydration center in ether lipids. Regardless of the phase state, cholesterol both in DMPC and 14:0 Diether PC vesicles, changed the distribution of water molecules decreasing the dipole relaxation of the lipid interphase generating an increase in the non-relaxable population. Above 10% Cholesterol/14:0 Diether PC ratio vesicles´ interphase present an environment around Laurdan molecules similar to that corresponding to ester PC.