INVESTIGADORES
ZANETTI Flavia Adriana
congresos y reuniones científicas
Título:
HETEROLOGOUS PRIME-BOOST IMMUNIZATION WITH RECOMBINANT PROTEINS AND MODIFIED VACCINIA ANKARA VECTOR AS A VACCINE STRATEGY AGAINST BABESIA bovis
Autor/es:
JOSÉ MANUEL JARAMILLO ORTIZ; MARÍA PAULA DEL MÉDICO ZAJAC; FLAVIA ADRIANA ZANETTI; MARÍA PAULA MOLINARI; MARÍA JOSÉ GRAVISACO; GABRIELA CALAMANTE; SILVINA WILKOWSKY
Lugar:
Cape Town
Reunión:
Conferencia; 8th International TTP and 12th Biennal STVM Conference.; 2014
Resumen:
The use of an attenuated live blood vaccine to immunize bovines against Babesia bovis is a current control measure in Argentina. However this vaccine has many disadvantages and can only be used safely in calves under 10 months of age. Protein-adjuvant vaccines are well known as inducers of antibodies meanwhile non-replicative viral vectors are able to express single or multiple antigens of interest in vivo and to elicit strong T-cell responses. The induction of both arms of the adaptive immune response is necessary for protection against B. bovis. In this work, we have obtained a recombinant modified vaccinia virus Ankara vector expressing a chimeric multi-antigen composed of B and T epitopes of three B. bovis immundominant proteins: Msa-2c, RAP-1 and Hsp20. This novel immunogen was evaluated in mice as a candidate vaccine in homologous and heterologous prime-boost immunizations with a cocktail of the 3 recombinant proteins. Humoral and cellular immune responses were evaluated by ELISA and flow cytometry to compare the immunogenicity induced by the different regimes. The best immune response in terms of high levels of specific IgG antibodies and secreted INFγ by CD4+ and CD8+ T cells was achieved with a prime of protein cocktail at day 0 and a boost of the recombinant virus at day 14. This is the first report in which a novel vaccine candidate was constructed based on a rationally designed multi-antigen and evaluated in a prime - boost regime, optimizing the immune response necessary for protection against bovine babesiosis.