Recombinant MVA expressing secreted glycoprotein D of BoHV-1 induces systemic and mucosal immunity in animal models

FERRER, MARÍA FLORENCIA; DEL MÉDICO ZAJAC, MARÍA PAULA; ZANETTI, FLAVIA ADRIANA; VALERA, ALEJANDRO RAFAEL; ZABAL, OSVALDO; CALAMANTE, GABRIELA

VIRAL IMMUNOLOGY

MARY ANN LIEBERT INC

Lugar: New York; Año: 2011 vol. 24 p. 331 - 339

0882-8245

Bovine herpesvirus-1 (BoHV-1) infection is distributed worldwide and the development of new tools to fight against this pathogen becomes extremely important. In this work a recombinant modified vaccinia virus Ankara (MVA) vector expressing the secreted version of glycoprotein D, MVA-gDs, was obtained and evaluated as a candidate vaccine. Firstly, the correct expression, antigenicity and N-glycosilation of glycoprotein D were confirmed by molecular techniques. Then, MVA-gDs was used as parenteral immunogen in BALB/c mice where a specific anti-gD humoral immune response was induced and maintained for seven months. Besides, two doses of MVA-gDs supplemented with cholera toxin delivered by intranasal immunization induced IgA anti-gD humoral immune responses in nasal and bronchiopulmonar washes as well as IgG anti-gD antibodies in serum samples. In order to evaluate the protection conferred by MVA-gDs immunization, a rabbit BoHV-1 challenge assay was performed. A shorter viral excretion period and a reduction on the number of animals shedding BoHV-1 was observed in the group immunized with recombinant MVA-gDs. In conclusion our data encourage further studies to evaluate MVA-gDs, alone or combined with other immunogens, as a candidate vaccine for BoHV-1.