GONZALEZ Ana Maria
congresos y reuniones científicas
Antibacterial interaction between extracts of Fridericia caudigera and Cuspidaria convoluta
TORRES C.A.; NUÑEZ M.B.; ZAMPINI I.C.; GONZALEZ A.M.
Congreso; III Reunión Internacional de Ciencias Farmacéuticas,; 2014
An interesting approach to the treatment of infectious diseases and prevention of the development of resistant microorganisms is the combination of antimicrobial agents. The aim of this work was to evaluate the antibacterial effect of the association between the extracts of Fridericia caudigera and Cuspidaria convoluta (Bignoniaceae) on several human pathogenic bacteria. The antimicrobial interaction between these antibacterial extracts was evaluated by the checkerboard method. The microorganisms used were Gram-positive bacteria: Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 29213, four clinical isolates of methicillin-sensitive Staphylococcus aureus and two methicillin-resistant clinical isolates of S. aureus; also Gram negative bacteria: Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853 and antibiotic-resistant clinical isolates of Enterobacter cloacae (two strains), Klebsiella pneumoniae, Morganella morganii, Proteus mirabilis and Pseudomonas aeruginosa. The fractional inhibitory concentration indices for all combinations were determined. Klebsiella pneumoniae and P. aeruginosa strains were resistant at used combinations. Nevertheless, synergism was demonstrated against the other Gram-negative bacteria. Furthermore, no antagonic effect was detected against Gram-positive bacteria, while synergism was found against four strains: E. faecalis, two ampicillin and methicillin resistant strains of S. aureus and ampicillin resistant S. aureus. The combinations had additive effect against the other three S. aureus strains and indifferent effect against S. aureus ATCC 29213. The results revealed that most of the combinations selected could efficiently inhibit the growth of tested bacterial strains at lower concentrations than those required for the individual extract. Considering the scarcity of plant extracts with good antibacterial activity against Gram-negative bacteria, these combinations offer good and promising prospects for the treatment of diseases caused by these bacteria in traditional medicine. .