In vitro and in vivo synergistic antitumor effect of thapsigargin and a penicillin derivative in murine melanoma cells

BELLIZZI, YANINA; CORNIER, PATRICIA G; DELPICCOLO CARINA; CAYROL MARIA F; CREMASCHI GRACIELA A; BOGGIAN, DORA B; MATA, ERNESTO G; BLANK VIVIANA C; ROGUIN LEONOR P

Congreso; Reunión Conjunta SAIC, SAFE, SAB, SAP. 2019; 2019

The triazolyl peptidyl penicillins (TAPs) are novel hybrids compounds having in their structure a penicillanic core linked to a peptide portion via a triazole group. In a previous study, we showed that the derivative containing the dipeptide Leu-Phe (TAP7f) activates an endoplamic reticulum (ER) stress response and induces apoptosis of murine B16-F0 melanoma cells. In this work, we first demonstrated the contribution of ER stress to the antitumor effect of TAP7f. When cell viability was determined after pre-incubating cells with an specific siRNA for CHOP, a protein involved in ER stress signaling, cell growth increased from 25±2% to 40±1% (p