Wood flour as carbon source for the enzymatic secretion of white rot fungi.

RODRIGUEZ, MARIA DANIELA; BARCHUK, LUCRECIA; CASTRILLO, MARIA LORENA; VELAZQUEZ, JUAN ERNESTO; KRAMER, GUSTAVO; ZAPATA, PEDRO DARIO; VILLALBA, LAURA LIDIA

Porto Alegre, RS.

Simposio; 8th Latin American Biodeterioration and Biodegradation Symposium.; 2013

Universidade Federal do Rio Grande do Sul, Oklahoma University,Universidade de São Paulo

Cellulose is the main component of plant cell wall, the most abundant polymer on the earth and a major renewableresource. Rot fungi, for their enzymatic capacity, are excellence organisms capable of degrading lignocellulosic material.The cellulase enzyme system consists of three main enzymes which act synergistically to degrade cellulose to glucose.One of these are endoglucanase, which acts on reducing ends of cellulose. Therefore, the objective was to assess theendoglucanases secretory capacity by white rot fungi native of Misiones (Argentina). Cellulolytic power was tested in 13strains, using pine and eucalyptus flour as carbon source. The strains were inoculated for duplicate in Mandels mediumwith carbon source at 1% W/V and 80rpm reciprocal shaking at 29°C, taking aliquots every 72 hours for 15 days.Enzymatic determination was performed by IUPAC technique on each aliquot, finding increased secretion from the 144hsof incubation. Elevated values endoglucanase activity were found using native strains, which are promising for cellulaseproduction, and on the other hand, wood flour will function as a accessible carbon source for the fungus and economicalin terms of productivity.