ENDOGLUCANASE SECRETION OF WHITE ROT FUNGI NATIVE OF MISIONES USING EUCALIPTUS SPP. SAWDUST AS SUBSTRATE

RODRIGUEZ, MARIA DANIELA; CASTRILLO, MARIA LORENA; KRAMER, GUSTAVO; VELAZQUEZ, JUAN ERNESTO; ZAPATA, PEDRO DARIO; VILLALBA, LAURA LIDIA

Puerto Iguazú

Congreso; Simposio Internacional de Materiales Lignocelulósicos.; 2013

Instituto de Materiales de Misiones (IMAM, UNaM-CONICET) junto con la Red Productos de Valor Agregado a partir de residuos agro y forestoindustriales (PROVALOR, CYTED)

Many white rot fungi have been studied for their ability to secrete xylanases, glucanases and cellulases. The main kinds of enzymes able to hydrolyse cellulose are: endo-1,4-B-glucanase, cellobiohydrolase and B-glucosidase. Some microorganisms, lacking of cellobiohydrolases, can replaced them by the endoglucanase progressive production, as they manage to combine mechanism action of both enzymes. The aim of the present work was to evaluate the endoglucanase activity of Phlebia spp. and Pycnoporus spp., native of the Misiones province grown on Eucaliptus spp. sawdust as substrate. The variables studied were: two culture temperatures and three different conditions of liquid/solid relationship. The incubation period was 32 days. The enzymatic activity was assayed by measuring glucose as the reducing sugar. The most favorable conditions correspond to the solid substrate culture using Phlebia spp.