EVALUATION OF SYNTHETIC AND SEMI- SYNTHETIC CULTURE MEDIA FOR ENDO-1,4-B- GLUCANASES SECRETION BY TRICHODERMA KONINGIOPSIS.

CASTRILLO, MARIA LORENA; BICH, GUSTAVO ANGEL; KRAMER, GUSTAVO; VELAZQUEZ, JUAN ERNESTO; RODRIGUEZ, MARIA DANIELA; ZAPATA, PEDRO DARIO; VILLALBA, LAURA LIDIA

Puerto Iguazú

Congreso; Simposio Internacional de Materiales Lignocelulósicos.; 2013

Instituto de Materiales de Misiones (IMAM, UNaM-CONICET) junto con la Red Productos de Valor Agregado a partir de residuos agro y forestoindustriales (PROVALOR, CYTED)

The actual demand of energy and the environmental concerns together with the reduced fossil fuel reserves haveplayed an important role to convert the second generation bioethanol production into an attractive researcharea. To convert lignocellulosic biomass to bioethanol the cellulosic components must be hydrolyzed tofermentable sugars. Trichoderma fungi secrete large amounts of enzymes of industrial interest such ascellulases, able to degrade holocellulose in the saccharification of lignocellulosic biomass. In this work weevaluated endo-1.4-B-glucanases enzymatic secretion of Trichoderma koningiopsis from Misiones province, insynthetic medium, with carboxymethylcellulose as carbon source; and semi-synthetic medium, with pine sawdustas carbon source. Higher values of endo-1.4-B-glucanases were reached when the semi-synthetic medium wasused. It could be concluded that pine sawdust seems to be a good candidate for utilization as carbon source inculture media aiming to obtain good enzyme secretion, being also an economic and easily available substrate.