Comparative study of single cultures and a consortium of white rot fungi for polychlorinated biphenyls treatment

BENITEZ, S.F.; SADAÑOSKI, M.A.; VELÁZQUEZ, J.E.; ZAPATA, P.D.; FONSECA, M.I.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2021

1364-5072

Aims: To evaluate the mycoremediation of polychlorinated biphenyls (PCBs)by either single cultures or binary consortia of Pleurotus pulmonarius LBM 105and Trametes sanguinea LBM 023.Methods and Results: PCBs tolerance, removal capacity, toxicity reductionand ligninolytic enzyme expression were assessed when growing single cultureand binary consortium of fungus in 217 mg l−1 of a technical mixture ofAroclor 1242, 1254 and 1260 in transformer oil. A decrease in tolerance andvariation in ligninolytic enzyme secretion were observed in PCB-amended solidmedia. Pleurotus pulmonarius LBM 105 mono-culture was able to remove upto 954% of PCBs, whereas binary consortium and T. sanguinea LBM 023could biodegrade about 55% after 24 days. Significant detoxification levelswere detected in all treatments by biosorption mechanism.Conclusions: Pleurotus pulmonarius LBM 105 in single culture had the bestperformance regarding PCBs biodegradation and toxicity reduction.Ligninolytic enzyme secretion changed in co-culture.Significance and Impact of the Study: The evaluation of PCBs bioremediationeffectiveness of basidiomycetes consortium in terms of PCB removal, toxicityand ligninolytic enzyme production to unravel the differences between usingindividual cultures or consortium has not been reported. The results from thisstudy enable the selection of P. pulmonarius LBM 105 mono-culture tobioremediate PCBs as it showed higher efficiency compared to binaryconsortium with T. sanguinea LBM 023 for potential decontamination of PCBcontaminatedtransformer oil.