TORRES PALAZZOLO carolina andrea
congresos y reuniones científicas
Effects of bioaccessible garlic compounds on cultured vascular smooth muscle cells from spontaneously hypertensive rats
Buenos Aires
Congreso; Reunión conjunta de las Sociedades de Biociencias y LXII Reunión anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2017
Institución organizadora:
Sociedades de Biología de Argentina: SAIC, SAIB, SAI, SAA, SAB, SAFE, SAFIS, SAH, SAP.
Several studies have associated a moderate consumption of garlic with improvements in health. The evidence suggests that its medicinal activities are mainly due to the organosulfur compounds (OSCs) garlic content. Garlic compounds have exhibited antioxidant activities and cardiovascular disease-protective properties. The remodeling of small arteries by excessive proliferation and migration of vascular smooth muscle cells (SMCs) is a phenomenon to consider, since it contributes to the development and complications of hypertension, one leading cause of morbidity and mortality globally. It seems that the mystery of OSCs and hypertension would be easy to elucidate; nevertheless, OSCs have shown to have different biological actions, potency and mechanisms in different cell lines and organ tissues. Moreover, only the bioavailable compounds would have bioefficacy. In this regard, previous works have identified a few OSCs in blood. For this study three bioaccessible OSCs, allyl mercaptan (AM), S-allyl cysteine (SAC) and 2-vinyl-4H-1,3-dithiin (2VD), were selected to evaluate their in-vitro effects on cultured SMCs isolated from mesenteric arteries of male spontaneously hypertensive rats. Cell proliferation, cell migration and intracellular oxidative stress were evaluated after cell exposure to OSCs expected quantities in plasma, 10 µg/L of AM and 2VD and 50 µg/L of SAC. The results of the proliferation test by MTT assay showed that 2VD reduced by 20% the proliferation compared to the control, conversely SAC enhanced the proliferation by 15%. Scrape-wound migration assay showed that 2VD and AM reduced the cell migration in similar ratios (30%), whereas SAC increased the migration by 30% compared to the control. Concerning to the intracellular ROS levels, the compounds showed different behaviors at the studied concentrations. While AM was an intracellular antioxidant, 2VD and SAC increased the oxidative stress. In conclusion, expected quantities of 2VD and AM in plasma, provided by garlic intake, were able to inhibit the migration of SMCs of spontaneously hypertensive rats. These compounds could help to protect the cardiovascular system from vascular remodeling produced by hypertension. Further studies will be needed to elucidate the specific mechanism of action to inhibit the SMCs migration and the role of its different antioxidant effects.