TORRES PALAZZOLO carolina andrea
congresos y reuniones científicas
Garlic phytochemicals and their interactions with blood components
RAMIREZ, DANIELA ANDREA; TORRES PALAZZOLO, CAROLINA; MANUCHA, WALTER; CASTRO, CLAUDIA; CAMARGO, ALEJANDRA BEATRIZ
Encuentro; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016
Sociedad de Biología de Cuyo
Numerous studies have related organosulfur compounds (OSCs) to in-vitro biological activities. However, in-vivo OSCs need to be bloodstream delivered before reaching their target sites. Previous studies have determined that only small amounts of OSCs could be detected in the blood. Never the less, it is unclear how the stability is and how the compounds are partitioned into the different blood fractions (plasma or formed elements). To clarify this issue, allicin, ajoene, 2-vinyldithiins (2VD), diallyl sulfide (DAS), diallyl disulfide (DADS) and diallyl trisulfide (DATS) were added to whole blood, plasma and deproteinized plasma. The analytes remaining amounts were extracted and quantified by HPLC-UV at different time intervals. Additionally, it was conducted an incubation of OSCs in whole blood by four hours, to evaluate the distribution rate in time. Results showed that OSCs in blood at zero time had low recovery values (4-8%). This might be due to reactions with sulfhydryl groups of plasmatic proteins and other molecules, such as glutathione present in erythrocytes. OSCs recovery values from plasma samples were higher than the previous ones (13-20%), this fact reinforces the theory that OSCs interact with the blood formed elements. Finally, OSCs added to deproteinized plasma showed high recovery values (87-98%); it could be related to the lack of proteins with sulfhydryl groups. As for the incubation of OSCs in whole blood, the analytes were found in the formed elements fraction in greater quantities than in plasma at all times; 2VD and DAS remained stable in this fraction during four hours. Moreover, all plasmatic amounts showed a significant decreasing trend between the first and the last time. OSCs distribution between the blood components showed to be an important factor to be considered in bioavailability studies.