Chips and sawdust substrates application for lignocellulolytic enzymes production by solid state fermentation

GIORGIO MARTIN; FONSECA MARIA ; TEJERINA MARCOS; RAMOS ANA BELEN

International Research Journal of Biotechnology

International Research Journals Publishing House

Lugar: New Jersey; Año: 2012

2141-5153

White rot fungi have an enzymatic system producing oxidative and hydrolytic enzymes that act on the degradation of the cell wall components. The abundance of sawdust and other forestry wastes in our region, due to an important forestry activity, represents an interesting ecological option. These surplus needs to be ecologically disposed. The purpose of this study was to compare wood chips and sawdust as substrates for the production of hydrolytic (endo-β-1,4-glucanase and 1,4-β-glucosidase) and oxidative (laccases and manganese peroxidase) enzymes secreted by five white rot fungi native from Misiones (Argentina) by solid state fermentation (SSF) We also evaluated the effect of the extraction method on enzyme recovery yield. Sawdust-SSF was proved to be the most suitable substrate for enzymes production. Endo-β-1,4-glucanase and laccase were the major enzymes secreted with both substrates. Peniophora sp. BAFC 633, T. villosa BAFC 2755 and P. sanguineus BAFC 2126 were the most efficient producers of both types of enzymes. G. applanatum BAFC 1168 strain F and C. versicolor f. antarcticus BAFC 266 produced mostly hydrolytic enzymes. Enzyme extraction was conducted in two steps to assure optimal recovery yield. A second extraction step was necessary to sawdust SSF samples.