Th17 cells are able to be expanded in response to specific bacterial and non-specific stimuli in patient with Chronic Granulomatous Disease.

BASILE J; COMAS D; ROMERO M; SCHIERLOH P; GIOVANNI D; GAILLARD M; YUNGBLUT C; DE LA BARRERA S; BEZRODNIK L

México DF

Congreso; II Meeting of LASID 2011; 2011

Latin American Society of Inmunodeficiencies

Chronic Granulomatous Disease CGD is a primary immunodeficiency involving lack function to produce reactive oxygen compounds by NADH oxidase complex affecting the ability of children to survive infections. CGD patients are characterized by showing granulomas and recurrent infectious events. Although, current therapies allow these patients an increased survival, develop symptoms of autoimmune and inflammatory diseases. What is more, these patients have blocked tryptophan metabolism as a consequence of their inborn inability to produce superoxide anion, which are required as cofactor for the first and rate-limiting enzyme of tryptophan catabolism through kynurenine pathway Indolamine 2,3 dioxigenase, favoring the imbalances between IL17 producing T cells Th17 and regulatory T cells Treg. The hypothesis of our study was to establish, among different stimulation cultures, the ability of CGD patients to develop a Th17 response. Peripheral mononuclear blood cells PMBC from 6 healthy donors and 10 CGD patients. 1 X-linked and 5 autosomal recessive, age range 5-18 years, all asymptomatics, were cultured alone or with OKT3IL2CD28 for 5 days, Staphylococcus enterotoxin B SEB for 16 hr, Mycobacteium tuberculosis Mtb H37Rv strain for 48 hr, IonomycinPMA for 5 days. IL17 expression was evaluated by flow cytometry on CD4, CD8 and gd T cells and results are expressed as percentage of IL17 cells within the CD4, CD8 and gamma/delta T subsets. IL17 CD4 T cells were induced by OKT3IL2CD28, H37Rv Mtb strain and Inomycin+PMA stimuli in heathly donnors and CGD patients.