Eventos tempranos subsiguientes a la privación de fósforo implican la modulacion del oxido nitrico.

GALATRO A; RAMOS-ARTUSO F; LIMA A; BATTHYÁNY DIGHIERO ; SANTA MARIA G.E.; SIMONTACCHI M

Corrientes

Congreso; XXX Reunión Nacional de Fisiología Vegetal; 2016

SAFV

 EARLY EVENTSFOLLOWING PHOSPHOROUS DEPRIVATION INVOLVE MODULATION OF NITRIC OXIDEEVENTOS TEMPRANOS AUBSIGUIENTES A LAPRIVACIÓN DE FÓSFORO IMPLICAN LA MODULACION DEL OXIDO NITRICO GALATRO Andrea1*, RAMOS ARTUSO1Facundo, LIMA Analía2, BATTHYÁNY DIGHIERO Carlos2, SANTAMARÍA Guillermo E. 3, SIMONTACCHI Marcela 1.1 INFIVE UNLP-CONICET Diagonal 113 y 61, La Plata, Argentina, 2 Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, Uruguay 3INTECH UNSAM-CONICET, Av. IntendenteMarino Km 8,200 CC 164, Chascomús, Buenos Aires, Argentina. *agalatro@ffyb.uba.arPhosphorus (P) is a criticalresource for plant growth being involved incellular metabolism, energy conversion, signal transduction and proteinactivity regulation. Nitric oxide (NO) and reactive oxygen species (ROS) playimportant physiological roles in signaling and acclimation to several stressconditions. In order to evaluate whether early events following P restrictioninclude an effect on NO levels and NO mediated responses, seven-day-old soybeanplants were exposed up to 48 h to a medium lacking P. As expected, total P-concentrationsignificantly decreased in roots and leaves after 48 h of P-deprivation.Chlorophyll content and quantum yield of photosystem II (FPSII) did not change in the first pair of leaves during that period.However, stomatal conductance was significantly affected as a consequence ofthe stress condition. Confocal laser microscopy (employing specific probes)unveiled a high NO level and ROS generation in P-deprived leaves. Moreover,analysis of nitrotyrosines by two-dimensional PAGE/western blot/Massspectrometry showed an increase in protein tyrosine nitration in leaves ofP-deprived plants. Additionally, a differential protein expression pattern,analyzed by nLC-ion trap MS, was observed in the leaves upon 24h of Prestriction. The results suggest the modulation of NO in early events relatedto P scarcity.