INVESTIGADORES
SANTA MARIA guillermo Esteban
artículos
Título:
Point mutations in the barley HvHAK1 potassium transporter lead to improved K+-nutrition and enhanced resistance to salt stress
Autor/es:
MANGANO S, SILBERSTEIN S, SANTA-MARÍA GE
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2008 vol. 582 p. 3922 - 3928
ISSN:
0014-5793
Resumen:
Abstract Members of group I KT-HAK-KUP transporters
play an important role in K+ acquisition by plant roots, a process
that is strongly affected by salt stress. A PCR-based random
mutagenesis approach on HvHAK1 allowed identification of
V366I and R591C substitutions, which confer enhanced K+-capture,
and improved NaCl, LiCl and NH4Cl tolerance, to yeast
cells. Improved K+-capture was linked to an enhanced Vmax. Results
reveal an intrinsic protective effect of K+, and assign an
important role to the 8th transmembrane domain, as well as
the C-terminus, in determining the maximum capacity for the
transport of K+ in KT-HAK-KUP transporters.