Intrarenal Renin Angiotensin System Components in Response to Tempol in Rats Fed with a High Salt Diet.

KOUYOUMDZIAN, N.M.; NATALIA L RUKAVINA MIKUSIC; GABRIEL CAO; SILVANA L DELLA PENNA; CHOI, M.R.; SUSANA GORZALCZANIC; BELISARIO E. FERNÁNDEZ; JORGE E TOBLLI; ROSON MI

Congreso; Congreso de Sociedad Argentina de Investigación Clínica (SAIC) ? Sociedad Argentina de Inmunología (SAI) ? Sociedad Argentina de Farmacología Experimental (SAFE); 2016

AIM: The aim of this study was to determine the effect of tempol in normal rats fed high salt on arterial pressure and the balance between antagonist components of the renal renin-angiotensin system (RAS).METHODS: Sprague-Dawley rats were fed with 8% NaCl (high-salt, HS) or 0.4% NaCl (normal-salt, NS) diet for 3 weeks, with or without tempol (T) (1mM, administered in drinking water). Mean arterial pressure (MAP), glomerular filtration rate (GFR), and urinary sodium excretion (UVNa) were measured. We evaluated Angiotensin II (Ang II), Angiotensin 1-7 (Ang 1-7), Angiotensin Converting Enzyme 2 (ACE2), Mas Receptor (MasR), Angiotensin Type 1 Receptor (AT1R) and Angiotensin Type 2 Receptor (AT2R) in renal tissues by immunohistochemistry. Immunostaining were performed by Image Pro Plus software analysis and expressed as integrated optical density (IOD) ± SEM in renal tissue.RESULTS: HS diet produced a slight but significant (p< 0.05) increase in MAP (NS: 92±3 vs HS: 108±3). HS group increased immunostaining of Ang II (NS: 1404±197 vs HS: 3424±146, p