RENAL NATRIURETRIC SYSTEMS ALTERATION IN FRUCTOSE-OVERLOADED HYPERTENSIVE RATS

PUYO AM; NICOLÁS KOUYOUMDZIAN; NATALIA L RUKAVINA MIKUSIC; ROUVIER E; CANTÚ SILVANA; LEE HJ; JORGE E TOBLLI; BELISARIO E. FERNÁNDEZ; MARCELO R. CHOI

París

Congreso; 26th European Meeting of Hypertension and Cardiovascular Protection; 2016

European Society of Hypertension

Abstract Body : ObjectiveMetabolic and hemodynamic changes induced by a high fructose diet could be associated with an impairment of renal natriuretic systems, which contributes to sodium retention and hypertension. There is little evidence about renal dopamine (DA) and type-A natriuretic peptide (ANP) interaction on blood pressure elevation in this model. The aim of this study was to evaluate both natriuretic systems in fructose-overloaded rats. Design and methodMale Sprague Dawley rats were randomly assigned: a) Controls (C, tap water to drink) and b) Fructose overloaded:(F, 10% w/v of fructose to drink), treated during 4, 8 and 12 weeks (n=8 rats/group/period). Urinary L-dopa and DA(HPLC); diuresis, creatinine and sodium were measured in 24 hour urine samples; and ANP (ELISA), triglycerides, glycemia, cholesterol, insulinemia, creatinine and sodium in plasma. Systolic blood pressure (SBP) was determined by tail-cuff and renal tissue protein expression of L-dopa transporter (LAT-2), D1R and NPR-A receptors by Western blot. ResultsFructose overload increased SBP (mmHg, C4: 121±8 vs. F4: 145±1*; C8: 130±4 vs. F8: 161±10#; C12: 133±5 vs. F12: 163±4#), which positively correlated (R2=0.78; p