INVESTIGADORES
GIRONACCI Mariela Mercedes
congresos y reuniones científicas
Título:
Effect of ghrelin on the expression and activity of tyrosine hydroxylase, an enzyme involved in hypertension
Autor/es:
N LONGO CARBAJOSA; CORRADI G; PERELLO M; GIRONACCI MM
Reunión:
Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017
Resumen:
Ghrelin, is involved in several metabolic and cardiovascular processes.In the brain, the receptor for ghrelin has been shown to localizein the main cardiovascular control centers in neurons of thenucleus tractus solitaries. Tyrosine hydroxylase (TH), the enzymethat catalyses the first and rate-limiting step in catecholaminesbiosynthesis, is involved in the development and maintenance ofhypertension. The aim of this study was to examine the effect ofghrelin on TH expression and activity. PC12 cells were incubatedin the presence or absence of 100 nM ghrelin during different timesand TH expression and phosphorylation (as an index of TH activity)were evaluated by Western-blot and immunofluorescence. Ourresults showed that ghrelin modulate TH expression in PC12 cellsin a time-dependent manner. TH expression showed an increaseof 30±5% above basal after 30 min incubation but a decrease of25±5% below basal after 24 h incubation in the presence of ghrelin(P