Ilex paraguariensis Reconfigure the Root Transcriptome and Metabolome in Response to Drought.

AVICO E,; ACEVEDO R. M.; RODRIGUEZ SALVADOR, A.; NUNES NESI, A.; RUIZ O.A.; SANSBERRO P

DUBLIN

Congreso; International Association for Plant Biotechnology Congress; 2018

IAPB

Ilex paraguariensis is a subtropical tree cultivated in south of Brazil,northeast of Argentina, and east of Paraguay. The occurrence of droughtepisodes during the active phase of the annual growth cycle leads with leafabscission and yield reduction. RNA-seq, gas chromatography-mass spectrometry,and spectrometry assays were used to elucidate a global understanding of howroots respond to dehydration. A total of 190,474 unigenes and 265,309transcripts were obtained from 74,911,111 pair-ends reads (2x100bp) from 6libraries. Among them, 30.6% were associated with a Gene Ontology (GO) term.The comparative analysis of transcriptomes between the control and eachstressed sample revealed that 8,046 transcripts were differentially expressed[DETs, FDR <0.001; log2(Fold Change) ≥ |1|]; among them, 3,020 wereup-regulated and 5,026 repressed by drought. GO functional enrichment revealsover-represented hormone signaling pathway, trans-acting elements families,cellular amino acids metabolism and development of root anatomical componentsinvolved in response to drought. Along with, 27 metabolites including aminoacids, carbohydrates, and organic acids varied their concentration in responseto stress. These results provide a comprehensive overview of how I.paraguariensis responds to dehydration at transcriptome and metabolomelevels which may help to understand molecular mechanisms associated withdrought response.