PYROSEQUENCING REVEALS CHANGES IN FUNGAL SOIL COMMUNITIES UNDER LOTUS TENUIS MONOCULTURE

NIEVA, A.S.; LLAMES M.E.; BAILLERES M.A.; MENENDEZ A.B.; RUIZ O.A.

CORDOBA

Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Flooding Pampa is the main region for the cattle production in Argentina. The soils from this area are mainly characterized chemical and physical limitations. Lotus tenuis is a naturalized forage legume in the Flooding Pampa able to grow under restrictive conditions, and its use is an alternative to increase the yields in beef production. is an agricultural practice consisting in removing competition from weeds and resident grass using herbicides, so the species of interest grows and fully develops, becoming the plant community into a L. tenuis monoculture grassland.Fungal communities could be affected by the change in the cover grass composition. Through sequencing by 454 FLX Roche technology of the internal transcribed spacer ITS1-ITS2, we studied the structure of fungal communities in different soils from flooding pampa, affected by this agronomical practice. Our results suggest that 6-7 years of application of this practice could modify the structure of fungal community evidenced by statistical differences between the structures of fungal community in soils affected by L. tenuis promotion compared with soils under natural grass cover. In addition, indicators reveal the presence of some fungi as the most representative taxa in this soils. Chemical variables like conductivity and Sodium are more related to the distribution of different fungi in this environment.