INVESTIGADORES
RUIZ oscar Adolfo
artículos
Título:
The R2R3-MYB TT2b and the bHLH TT8 genes are the major regulators of proanthocyanidin biosynthesis in Lotus species
Autor/es:
ESCARAY F.J.; PASSERI V.; PEREA GARCIA, A; ANTONELLI C.J.; DAMIANI F.; RUIZ O.A.; PAOLOCCI F
Revista:
PLANTA
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2017 vol. 246 p. 243 - 261
ISSN:
0032-0935
Resumen:
Proanthocyanidins (PAs), also called condensed tannins, are polymeric flavonoids based on catechin or epicatechin starter units. PAs are among the compounds that strongly affect the nutritional value of forage legumes. Moderate amount of these metabolites prevent bloat in ruminant animals. Since the major forage legumes, such as alfalfa and clovers, lack PAs in edible tissues, there is a pressing need to engineering the PA trait in their herbage as a means to improve the ecological and economical sustainability of cattle production system. Progresses on the understanding of genetic determinants controlling PA biosynthesis and accumulation have been mainly made studying mutants of Arabidopsis and Medicago truncatula, two model species that synthetize epicatechin based PAs in the seed coat. To gain a leap forward into the genetic control of PA biosynthesis in leaves of forage legumes here we took advantage of hybrids between Lotus corniculatus, with a high levels of PAs in the leaves, and L. tenuis, with no PAs in these organs, and relative F2 progeny, to identify among candidate PA regulatory and transporter genes whose that co-segregate with this trait. We show that the levels of leaf PAs significantly correlate with the expression of MATE1, the putative transporter of glycosylated PA monomers, and, among the candidate regulatory genes, with the expression of TT2b (MYB) and TT8 (bHLH). Present study adds new players and highlights differences in the regulation of PA biosynthesis in our Lotus genotypes with respect to model species. This information are crucial to build bloat safe forage legumes.