INVESTIGADORES
RUIZ oscar Adolfo
artículos
Título:
OVEREXPRESSION OF THE ARGININE DECARBOXYLASE GENE IMPROVES TOLERANCE TO SALT STRESS IN LOTUS TENUIS PLANTS
Autor/es:
ESPASANDIN, F.D.; CALZADILLA P.I.; MAIALE S.J.; RUIZ O.A.; SANSBERRO P.A.
Revista:
JOURNAL OF PLANT GROWTH REGULATION
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2018 vol. 37 p. 156 - 165
ISSN:
0721-7595
Resumen:
To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild type and transgenic lines harbouring the pRD29A::oat ADC (arginine decarboxylase, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L-1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8-fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/K+ ratio. The analysis of the gene expression, enzymatic activities and hormone metabolism suggest a crosstalk between polyamines and abscisic acid in response to salinity via the modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51) at the transcriptional level.