INVESTIGADORES
RUIZ oscar Adolfo
artículos
Título:
Simultaneous determination of S-adenosylmethionine and S-adenosylhomocysteine by capillar zonal electrophoresis
Autor/es:
PAÑAK KARINA, GIORGIERI SERGIO, LUIS DIAZ AND OSCAR RUIZ.
Revista:
ELECTROPHORESIS
Referencias:
Año: 1997 vol. 18 p. 2047 - 2049
ISSN:
0173-0835
Resumen:
A reproducible, rapid procedure for the simultaneous quantitative separation of S-adenosylmethionine and S-adenosylhomocysteine by capillary zone electrophoresis has been developed. Separations were performed by using an uncoated capillary of 60 cm effective length and 50 m ID, 40 mM sodium phosphate buffer, pH 2.50, as background electrolyte solution, and 30 kV. On-line detection was carried out at 254 nm. Under the conditions selected we resolved a standard solution containing S-adenosylmethionine and S-adenosylhomocysteine in a run time shorter than 8 min. A mass detection limit in the range of 10 fmol was achieved. Adenosyl-L-methionine, S[methyl-3H] has also been assayed under the same experimental conditions. Other related compounds did not show interference, including those derived from the hydrolysis of S-adenosylmethionine. The present method allows simultaneous determination of these compounds, which play an important role in many microbiological and enzymatic research studies.