INVESTIGADORES
RUIZ oscar Adolfo
artículos
Título:
Polyamine catabolism is involved in response to salt stress in soybean hypocotyls
Autor/es:
CAMPESTRE M.P.; BORDENAVE C.; ORIGONE A.; MENÉNDEZ, A. B.; RUIZ O.A.; RODRIGUEZ, A.A.; MAIALE, S.
Revista:
JOURNAL OF PLANT PHYSIOLOGY
Editorial:
ELSEVIER GMBH
Referencias:
Lugar: Kusterdingen; Año: 2011 vol. 168 p. 1234 - 1240
ISSN:
0176-1617
Resumen:
The possible relationship between polyamine catabolism mediated by copper-containing amine oxidase and the growth of hypocotyl from soybean plants exposed to NaCl has been studied. Salt treatment reduced values in all growth parameters in the hypocotyls. In vitro, copper-containing amine oxidase activity was up to 77-fold higher than that of polyamine oxidase. This enzyme preferred cadaverine over putrescine and it was active even under the saline condition. On the other hand, saline stress increased spermine and cadaverine levels and in vivo copper-containing amine oxidase activity in the elongation zone of hypocotyls. The last effect was negatively modulated by the addition of diaminoguanidine. In turn, plants treated with diaminoguanidine showed a significant reduction of reactive oxygen species in the elongation zone even in the saline condition. In addition, plants grown in cadaverine-amended culture medium showed increased hypocotyl length either in saline or control conditions. The cadaverine-induced hypocotyl elongation of untreated and salt-treated plants was abolished in presence of the inhibitor diaminoguanidine. These results suggest that copper-containing amine oxidase activity may be contributing partially to hypocotyl growth under saline stress, through hydrogen peroxide produced by polyamine catabolism.