INVESTIGADORES
MEDINA ricardo Daniel
artículos
Título:
Plant recovery of cryopreserved apical meristem-tips of Melia azedarach L. using encapsulation/dehydration and assessment of their genetic stability
Autor/es:
A SCOCCHI; M FALOCI; RICARDO DANIEL MEDINA; S OLMOS; LA MROGINSKI
Revista:
EUPHYTICA
Editorial:
Springer Netherlands
Referencias:
Lugar: Dordrecht, The Netherlands; Año: 2004 vol. 135 p. 29 - 38
ISSN:
0014-2336
Resumen:
A cryopreservation process using encapsulation/dehydration technique was set up for apical meristem-tips of in vitro plantlets of ?paradise tree? (Melia azedarach L. var. gigantea, clone ?El dorado?). Apical meristem-tips were cultured for one day on MS basal medium with 2 μM BA and 0.5 μM IBA and encapsulated with 3% sodium alginate. The highest shoot proliferation rate after cryopreservation was obtained when encapsulated apical meristem-tips were pregrown for 3 days in liquid medium with 0.5, 0.75 and 1 M of sucrose for 24 hours progressively, desiccated for 5 hours with silicagel followed by rapid or slow cooling. Survival after freezing in liquid nitrogen ranged between 67?83% and shoot proliferation ranged between 43?60%. This cryopreservation treatment preserved genetic stability, when it was evaluated using the electrophoretic patterns of nine isozyme systems and RAPD profiles.
