INVESTIGADORES
RODRIGUEZ maria eugenia
artículos
Título:
Characterization of a-rhamnosidase activity from a Patagonian Pichia guilliermondii wine strain
Autor/es:
RODRÍGUEZ M.E; LOPES C.A.; VALLES S.; CABALLERO A.C.
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 109 p. 2206 - 2213
ISSN:
1364-5072
Resumen:
Aims: The purpose of this study was to characterize the a-l-rhamnosidase of Pichia guilliermondii NPCC1053 indigenous wine strain from North-Patagonian region. Methods and Results: The optimization of yeast culture conditions was carried out and the effects of oenological parameters on a-l-rhamnosidase activity were evaluated. Additionally, the effect of direct contact with must and wine on a-l-rhamnosidase activity was assayed. This strain showed an intracellular inducible a-l-rhamnosidase activity. This enzyme was active at pH, glucose and SO2 concentrations usually found at the beginning of the fermentation as well as retained high levels of activity after 24 h of incubation in must. Furthermore, P. guilliermondii a-l-rhamnosidase was able to release monoterpenols and alcohols from grape glycosidic extracts. Conclusions: The a-l-rhamnosidase belonging to P. guilliermondii indigenous wine yeast strain showed mainly an intracellular location and evidenced interesting oenological characteristics. Significance and Impact of the Study: This study contributes to the knowledge of a-l-rhamnosidases from yeast origin because at present, there are few reports about this enzymatic activity in these micro-organisms. In addition, this work is relevant to the regional wine industry considering that this enzyme could be used in the production of more aromatic young wines.