IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Male and female reproductive effects on gonads, germ cells and early embryos produced by parental alcohol ingestion, in the mouse model
Autor/es:
CEBRAL E
Lugar:
Cordoba
Reunión:
Congreso; IXI LASBRA International Meeting; 2019
Resumen:
Chronic alcohol consumption affects the male and female hypothalamic-pituitary-gonadal axis. However, the effects of moderate-low alcohol intake at gonadal-germinal level and its impact on early embryonic development have not been fully described. Previously, we demonstrated in mouse that chronic administration of 5-10% alcohol in drinking water, decreases the ovulation, alters the oocyte quality and reduces the fertilization rate (IVF); while in the male, only the moderate alcohol concentration (10%) induced teratozoospermia, without changes in the sperm functionality and fertilization. However, paternal 10% alcohol consumption induced delayed preimplantation development from day 3 to 7 of culture, whereas the administration of alcohol 5% to murine females affected the development from the postzygotic stages. Recently, we focused on the gonad-germinal impact of moderate semichronic intake (15 days) of alcohol (10, 15%) in the mouse (Blood alcohol concentration: 15?60 mg/dL, ethanol consumption: 18-30 g/kg/day, 24-30 % EDC). In 80% of females treated with 10% alcohol, the cyclicity was altered, evidenced by permanent diestrus and lower estrus frequency. After intake, the number of preantral-antral follicles was significantly reduced compared to controls. Although ovulatory induction (5 IU eCG/hCG) restores folliculogenesis, high spontaneous parthenogenetic activation and increased frequency of oocytes with abnormal metaphase II remained observed. After semichronic alcohol administration, the testicular seminiferous tubules had reduced epithelial diameter, disorganization, vacuolization, apoptosis and altered intercellular adhesion. In addition, we recently demonstrated that, although the acrosomal reaction and hyperactivation of motility decreased after semichronic treatment, during IVF timing (2.5, 3.5 and 4.5 h) increased percentages of fertilized oocytes, of in vitro induced-decondensed sperm heads and sperm morphologically abnormalities were observed. These results suggest that short-term moderate alcohol consumption in male and female mice affects the fertility and may potentially lead to ulterior early embryonic development impairment, hypothesis that now are under studying.