IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Banco de Glomeromycota In vitro (BGIV): an in vitro collection of arbuscular mycorrhizal fungi from Argentina
Autor/es:
SCORZA, MARIA V.; STATELLO, MARINA; PERGOLA, MARIANA; GODEAS, ALICIA M.; SILVANI, VANESA A.; COLOMBO, ROXANA P.
Lugar:
Bariloche
Reunión:
Simposio; II Simposio Internacional Simbiosis Micorrícica en Sudamérica; 2019
Institución organizadora:
INIBIOMA
Resumen:
Due to the obligate biotrophic nature of the arbuscular mycorrhizal (AM) fungi, the traditional method to propagate them is through the inoculation of plants growing in sterile substrates under greenhouse conditions. This technique has two important disadvantages: the required space and the impossibility to isolate the pot cultures from others microorganisms. Another way to propagate AM fungi, involves the in vitro cultivation by using transformed roots growing in Petri plates with minimal medium. This in vitro technique allows the symbiosis establishment and the development of high amounts of spores and mycelia in much less space, high purity and monospecificity conditions. The Banco de Glomeromycota in vitro (BGIV), created in 2003 at the Lab. Microbiología del Suelo (FCEyN-UBA), and registered in the World Data Center of Microorganisms in 2010, seeks to multiply the germplasm of AM fungi in association with Transformed carrot roots (Ri T-DNA Daucus carota) under in vitro conditions. This collection contributes to the knowledge on the biodiversity of AM fungi from agricultural or natural environments, and the ex-situ conservation of this important biological resource, thus facilitating sustainable use of their germplasms. The BGIV collection propagate and maintain native AM fungal strains isolated from different ecosystems of Argentina (Salta, Córdoba, La Rioja, Buenos Aires and C.A.B.A.). Nowadays, 40 strains of diverse species (Rhizoglomus, Glomus, Gigaspora, Claroideoglomus) are cultured and characterized by morphological and molecular techniques (SSU 18S). Recently, theFederación Latinoamericana de Colecciones and the Asosiación Argentina de Microbiología have also incorporated the BGIV to their institutions (SI-62 and SCCM 026, respectively). The BGIV has a web page (www.bgiv.com.ar) with a detailed on line catalogue of AM fungal strains, as well as, a gallery of photos and video, research works carried out with strains of the collection and links to other pages of interest.