IBBEA   24401
INSTITUTO DE BIODIVERSIDAD Y BIOLOGIA EXPERIMENTAL Y APLICADA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Diclofenac and caffeine induce hepatic oxidative stress in Astyanax altiparanae (Teleostei: Characidae) in acute and subchronic exposures
Autor/es:
MUÑOZ-PEÑUELA M.; TOLUSSI, C.E.; SOUZA BRANCO G; GUIMARAES MOREIRA, R.; DAL´ OLIO GOMES A.; LO NOSTRO, F.
Lugar:
Dublin
Reunión:
Congreso; SETAC Europe 30th annual meeting; 2020
Institución organizadora:
SETAC
Resumen:
The number of studies about aquatic contamination by pharmacological compounds and their toxic effects on the aquatic fauna has been increasing. Among them, we highlight diclofenac (DCF) and caffeine (CAF) that induce oxidative stress in fish. However, most of the studies have been carried out in temperate regions. Therefore, the aim of this study was to evaluate the effects of DCF and CAF, isolated and combined, on the antioxidant system in the liver of adult males of Astyanax altiparanae, a neotropical teleost, in acute (96 h) and subchronic (14 d) exposures. Four groups were established: control (CTR), DCF, CAF and a mixture (DCF+CAF). For the acute bioassay, 10% of the LC50-96 h value for this species was used: 3.08 mg.L-1 for DCF, 9.59 mg.L-1 for CAF, and the same concentrations were combined in the mixture. For the subchronic bioassay, the following concentrations were used: 0.4 µg.L-1 for DCF, 27.5 µg.L-1 for CAF, and the same concentrations when combined. The activity of oxidative stress enzymes as superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and glutathione S-transferase (GST) were measured, as well as lipid peroxidation. In the acute bioassay, DCF treatment decreased the activity of SOD and GPx compared to the CTR, whereas CAF treatment decreased CAT activity. Regarding the mixture (DCF+CAF) treatment, SOD and GST activity decreased when compared to CTR fish. In the subchronic bioassay, DCF alone inhibited GPx, CAT and GST activities, and DCF+CAF triggered the inhibition of SOD, CAT and GST activity. Unlike the acute bioassay, in the subchronic exposure CAF did not change the antioxidant enzymes, which may indicate that at low concentrations, this compound has no toxicity in this species during 14 d. Although in both, acute and subchronic bioassays, DCF decreased the activity of most antioxidant enzymes analyzed, this profile was not enough to generate lipid peroxidation during subchronic bioassay. In the acute bioassay, fish exposed to DCF showed greater damage compared to CTR. In conclusion, DCF separated or combined with CAF, induced hepatic oxidative stress in A. altiparanae exposed to high concentrations for short periods as well as low concentrations for a longer time. However, CAF seems to affect CAT at high concentration even for short periods. Therfore, under subchronic exposure even with lower enzymatic activities there was no hepatic lipoperoxidation, while under acute exposure a hepatic damage was observed.